Incubation:Article Title: Anxiety-like behavior is modulated by a discrete subpopulation of interneurons in the basolateral amygdala
Article Snippet: .. Subsequently, sections were exposed for 60 min to goat anti-rabbit biotin-conjugated IgG, (1:500 dilution in incubation solution, Vector Laboratories) and for 60 min to avidin-biotin-horseradish peroxidase (ABC-elite, 1:1,000 dilution in PBS; Vector Laboratories). .. Next the sections were exposed to tyramide amplification solution (1:250 dilution; PerkinElmer, Waltham MA), and to DTAF-conjugated streptavidin (1:100 dilution; Jackson Immunoresearch, West Grove PA, for GABA and CCK-8 dual-labeling) or Cy3-conjugated streptavidin (1:100 dilution; Jackson Immunoresearch, for NPY dual-labeling).
Article Title: Unusual dopamine-mediated regulation of the phototransduction in lamprey compared to jawed vertebrates
Article Snippet: The sections were then incubated with either the primary rabbit anti-D1 dopamine receptor antibody (Abcam; dilution 1:300) or the primary rabbit anti-D2 receptor antibody (Millipore; dilution 1:200) in a 2% blocking solution for 48 hours at 4 °C. .. After washing in PBST for 40 minutes, the sections were incubated for one hour in PBST with goat anti-rabbit biotin-conjugated IgG (Vector Laboratories, Inc.) at a dilution of 1:600. .. After washing in PBS, the sections were incubated for 1 hour in a streptavidin-peroxidase solution at a dilution of 1:1000 (Sigma, USA) in PBS.
Article Title: Netrin-1 Is a Novel Myelin-Associated Inhibitor to Axon Growth
Article Snippet: .. Primary antibody binding was detected with horse anti-goat, horse antimouse, or goat anti-rabbit biotin-conjugated IgG (1:200; Vector Laboratories, Burlingame, CA) followed by incubation with biotin– avidin-peroxidase complex (1:100 in TBS; Elite kit; Vector Laboratories) and Alexa Fluor 488 or Alexa Fluor 594-conjugated streptavidin (1:200 in TBS; Invitrogen), or by development in a diaminobenzidine reaction. .. The following antibodies were used: goat anti-netrin-1 (N18) IgG (4 g/ml for light-level IHC, SC-9291; Santa Cruz Biotechnology); mouse anti-Neurofilament (200 kDa) IgG1, MAB5262, clone RT97 (25 ng/ml for light-level IHC; Millipore, Temecula, CA); mouse anti-NeuN (IgG), MAB 377 (5 g/ml; Millipore); rabbit anti-CGRP, serum, AB1971 (1: 8000 for light-level IHC, 1:2500 for fluorescent IHC; Millipore); mouse anti-adenomatous polyposis coli (APC) mature oligodendrocyte marker IgG, Ab-7 (2 g/ml; Oncogene, San Diego, CA); rabbit anti-glial fibrillary acidic protein (GFAP), purified Ig fraction (1:1000 for light-level IHC, Z0334; Dako, High Wycombe, UK); goat anti-green fluorescent protein IgG (666 ng/ml for fluorescent IHC; Rockland Immunochemicals, Gilbertsville, PA).
Article Title: Differences in the Effect of Dopamine on the Phototransduction Between Lampreys and Jawed Vertebrates
Article Snippet: The sections were incubated with either the primary rabbit anti-D1 dopamine receptor antibody (Abcam; dilution 1:300) or the primary rabbit anti-D2 receptor antibody (Millipore; dilution 1:200) in a 2% blocking solution for 48 h at 4 °C. .. After washing in PBST for 40 min, the sections were incubated for one hour in PBST with goat anti-rabbit biotin-conjugated IgG (Vector Laboratories, Inc., Newark, CA, USA) at a dilution of 1:600. .. After washing in PBS, the sections were incubated for 1 h in a streptavidin-peroxidase solution at a dilution of 1:1000 (Sigma, USA) in PBS.
Article Title: Differences in the Effect of Dopamine on the Phototransduction Between Lampreys and Jawed Vertebrates.
Article Snippet: The sections were incubated with either the primary rabbit anti-D1 dopamine receptor antibody (Abcam; dilution 1:300) or the primary rabbit anti-D2 receptor antibody (Millipore; dilution 1:200) in a 2% blocking solution for 48 h at 4 ◦C. .. After washing in PBST for 40 min, the sections were incubated for one hour in PBST with goat anti-rabbit biotin-conjugated IgG (Vector Laboratories, Inc., Newark, CA, USA) at a dilution of 1:600. .. After washing in PBS, the sections were incubated for 1 h in a streptavidin-peroxidase solution at a dilution of 1:1000 (Sigma, USA) in PBS.
Avidin-Biotin Assay:Article Title: Anxiety-like behavior is modulated by a discrete subpopulation of interneurons in the basolateral amygdala
Article Snippet: .. Subsequently, sections were exposed for 60 min to goat anti-rabbit biotin-conjugated IgG, (1:500 dilution in incubation solution, Vector Laboratories) and for 60 min to avidin-biotin-horseradish peroxidase (ABC-elite, 1:1,000 dilution in PBS; Vector Laboratories). .. Next the sections were exposed to tyramide amplification solution (1:250 dilution; PerkinElmer, Waltham MA), and to DTAF-conjugated streptavidin (1:100 dilution; Jackson Immunoresearch, West Grove PA, for GABA and CCK-8 dual-labeling) or Cy3-conjugated streptavidin (1:100 dilution; Jackson Immunoresearch, for NPY dual-labeling).
Article Title: Netrin-1 Is a Novel Myelin-Associated Inhibitor to Axon Growth
Article Snippet: .. Primary antibody binding was detected with horse anti-goat, horse antimouse, or goat anti-rabbit biotin-conjugated IgG (1:200; Vector Laboratories, Burlingame, CA) followed by incubation with biotin– avidin-peroxidase complex (1:100 in TBS; Elite kit; Vector Laboratories) and Alexa Fluor 488 or Alexa Fluor 594-conjugated streptavidin (1:200 in TBS; Invitrogen), or by development in a diaminobenzidine reaction. .. The following antibodies were used: goat anti-netrin-1 (N18) IgG (4 g/ml for light-level IHC, SC-9291; Santa Cruz Biotechnology); mouse anti-Neurofilament (200 kDa) IgG1, MAB5262, clone RT97 (25 ng/ml for light-level IHC; Millipore, Temecula, CA); mouse anti-NeuN (IgG), MAB 377 (5 g/ml; Millipore); rabbit anti-CGRP, serum, AB1971 (1: 8000 for light-level IHC, 1:2500 for fluorescent IHC; Millipore); mouse anti-adenomatous polyposis coli (APC) mature oligodendrocyte marker IgG, Ab-7 (2 g/ml; Oncogene, San Diego, CA); rabbit anti-glial fibrillary acidic protein (GFAP), purified Ig fraction (1:1000 for light-level IHC, Z0334; Dako, High Wycombe, UK); goat anti-green fluorescent protein IgG (666 ng/ml for fluorescent IHC; Rockland Immunochemicals, Gilbertsville, PA).
Binding Assay:Article Title: Netrin-1 Is a Novel Myelin-Associated Inhibitor to Axon Growth
Article Snippet: .. Primary antibody binding was detected with horse anti-goat, horse antimouse, or goat anti-rabbit biotin-conjugated IgG (1:200; Vector Laboratories, Burlingame, CA) followed by incubation with biotin– avidin-peroxidase complex (1:100 in TBS; Elite kit; Vector Laboratories) and Alexa Fluor 488 or Alexa Fluor 594-conjugated streptavidin (1:200 in TBS; Invitrogen), or by development in a diaminobenzidine reaction. .. The following antibodies were used: goat anti-netrin-1 (N18) IgG (4 g/ml for light-level IHC, SC-9291; Santa Cruz Biotechnology); mouse anti-Neurofilament (200 kDa) IgG1, MAB5262, clone RT97 (25 ng/ml for light-level IHC; Millipore, Temecula, CA); mouse anti-NeuN (IgG), MAB 377 (5 g/ml; Millipore); rabbit anti-CGRP, serum, AB1971 (1: 8000 for light-level IHC, 1:2500 for fluorescent IHC; Millipore); mouse anti-adenomatous polyposis coli (APC) mature oligodendrocyte marker IgG, Ab-7 (2 g/ml; Oncogene, San Diego, CA); rabbit anti-glial fibrillary acidic protein (GFAP), purified Ig fraction (1:1000 for light-level IHC, Z0334; Dako, High Wycombe, UK); goat anti-green fluorescent protein IgG (666 ng/ml for fluorescent IHC; Rockland Immunochemicals, Gilbertsville, PA).
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